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Combining 3D single-molecule tracking with super-resolution microscopy, K. Lasker observed the trajectory and co-localization of individual proteins in membraneless compartments in asymmetrically dividing Caulobacter crescentus bacteria at each pole of the cell during cell division.
E. Nehme et al. trained a neural network to localize multiple emitters with overlapping tetrapod point spread functions over a large axial range and then to design the optimal point spread function for such a multi-emitter case. This approach was used to image mitochondria and telomeres in cells.
Using 3D single-particle tracking to map the transport of self-propelled Janus nanoparticles in an inverse opal matrix, H. Wu et al. found that these nanoswimmers escaped from cavities more than an order faster than expected when compared with Brownian particles.
Using 3D single-particle tracking with nanobody arrays and light sheet, to gently track live cell multiple chromosomal loci throughout the mammalian cell nucleus, Gustavson et al. identify a method to advance fundamental understanding of chromatin dynamics and how it is altered during disease progression.
A.R. Roy et al. used a double-helix point spread function to enable 3D single-molecule super-resolution localization microscopy at the nano-bio interface, determining the relative spatial position and distribution of proteins with 10-20 nm precision.
Tilted light sheet microscopy with 3D point spread functions (TILT3D) combines a novel, tilted light sheet illumination strategy with long axial range point spread functions (PSFs) for low-background, 3D super-localization of single molecules as well as 3D super-resolution imaging in thick cells
Carr et al. quantify the dynamics of T- cell receptors using extended depth 3D super-resolution imaging with Double Helix. This creative study combines the high-depth, high-precision Double Helix with multi-plane super-resolution imaging and single particle tracking to visualize the cell surface of entire live Eukaryotic cells.
Using the SPINDLE®, Wang et al showed that combining the Double Helix-PSF with variable-angle illumination epifluorescence microscopy (aka pseudo-TIRF), the signal to noise ratio, and hence, the localization precision of point emitters can be improved up to five-fold. This study will allow users of the SPINDLE™ to improve the accuracy of their image and track reconstructions with their current microscope set-up.
Jain and Wheeler et al. utilize the SPINDLE™ to visualize and characterize previously unseen stress granule cores. Stress granules are sub-cellular aggregates associated with ALS and Dementia. This ground-breaking study proposes a new model for the formation and dynamics of stress granules based on proteomic studies, in vitro isolation, and in vivo 3D Double Helix super-resolution imaging.
Gahlmann et al. utilize Double Helix with super-resolution to reconstruct three dimensional, three color super-resolution images of live bacteria using fluorescent proteins and a lipophilic dye. This innovative study quantitatively describes the sub-cellular structure of Caulobacter crescentus bacteria.
Grover et al. describe the Double Helix SPINDLE method and utilize super-resolution microscopy to reconstruct sub-diffraction 3D images of microtubules. This influential paper utilizes the Double Helix point spread function for high precision 3D sub diffraction super-resolution imaging.