3D single-molecule super-resolution microscopy with a tilted light sheet

Tilted light sheet microscopy with 3D point spread functions (TILT3D) combines a novel, tilted light sheet illumination strategy with long axial range point spread functions (PSFs) for low-background, 3D super-localization of single molecules as well as 3D super-resolution imaging in thick cells

NATURE 2018

APPLICATION NOTE

3D Super-Resolution Imaging and Tracking Unveils Receptor Dynamics of T-Cells

Carr et al. quantify the dynamics of T- cell receptors using extended depth 3D super-resolution imaging with Double Helix. This creative study combines the high-depth, high-precision Double Helix with multi-plane super-resolution imaging and single particle tracking to visualize the cell surface of entire live Eukaryotic cells.

BIOPHYSICAL JOURNAL 2017

3D Localization and Tracking Using the SPINDLE™ with Variable-Angle Illumination Epifluorescence Microscopy

Using the SPINDLE®, Wang et al showed that combining the Double Helix-PSF with variable-angle illumination epifluorescence microscopy (aka pseudo-TIRF), the signal to noise ratio, and hence, the localization precision of point emitters can be improved up to five-fold. This study will allow users of the SPINDLE™ to improve the accuracy of their image and track reconstructions with their current microscope set-up.

APPLIED PHYSICS LETTERS 2017

3D Super-Resolution Uncovers RNA Stress Granule Cores

Jain and Wheeler et al. utilize the SPINDLE™ to visualize and characterize previously unseen stress granule cores. Stress granules are sub-cellular aggregates associated with ALS and Dementia. This ground-breaking study proposes a new model for the formation and dynamics of stress granules based on proteomic studies, in vitro isolation, and in vivo 3D Double Helix super-resolution imaging.

CELL JANUARY 2015

APPLICATION NOTE

Multi-Color 3D Super-Resolution of Live Bacteria

Gahlmann et al. utilize Double Helix with super-resolution to reconstruct three dimensional, three color super-resolution images of live bacteria using fluorescent proteins and a lipophilic dye. This innovative study quantitatively describes the sub-cellular structure of Caulobacter crescentus bacteria.

NANOLETTERS FEBRUARY 2013

3D Super-Resolution Imaging of Microtubule Structure

Grover et al. describe the Double Helix SPINDLE method and utilize super-resolution microscopy to reconstruct sub-diffraction 3D images of microtubules. This influential paper utilizes the Double Helix point spread function for high precision 3D sub diffraction super-resolution imaging.

OPTICS EXPRESS 2013

Video: Super Resolution and the Double Helix Point Spread Function, W.E. Moerner Nobel Laureate Professor W.E. Moerner describes the Double Helix Light Engineering™ technology