Phase masks

Phase masks

Upgrade to 3D with engineered PSFs

Our comprehensive library of interchangeable Deep Focus and 3D phase masks are designed to match your imaging depth and system requirements for life sciences or inspection applications.

  • DHO phase masks transform a microscope’s native point spread function into an engineered point spread function (ePSF) that eliminates the typical tradeoff between depth capture and resolution
  • Choose between our Deep Focus and 3D families of phase masks, depending on your application needs
  • Instantaneously extend the depth of field up to 6x that of conventional optics with 2.5D Deep Focus phase masks - perfect for spatial omics, high-throughput cell or tissue screening, high-throughput inline or offline inspection, inspection of curved or out-of-plane samples, and more
  • Instantaneously extend the depth of field up to 24x that of conventional optics with 3D phase masks and capture 3D point clouds with isotropic precision - perfect for 3D single-molecule localization microscopy (SMLM), 4D single-particle tracking, 3D smFISH, CLEM, 3D surface metrology, and more
  • Optimize data quality with a phase mask that matches your required depth range, emission wavelength, signal over background, and microscope optics

Select a phase mask from our library

Double helix
Single helix
Deep focus
Multicolor
Custom order

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3DTRAX® in action

3D SMLM

3D SMLM

Reconstruct entire 3D structures with molecular precision

Deep Focus smFISH

Deep Focus smFISH

See entire structures in focus with 5x fewer axial slices

4D live-cell tracking

4D live-cell tracking

Capture time evolution of molecular targets in 3D

Spatial omics counting

Spatial omics counting

Rapidly & accurately count labeled molecules through a 3D volume

3DTRAX® modules

3D super resolution

Turn raw images into super-resolved 3D point clouds

3D PSF localization
Drift correction
Accurate photon counting
Cluster analysis

4D particle tracking

Track motion of particles in dynamic systems over time & space

3D PSF localization
Track segmentation
Accurate photon counting
Drift correction

2.5D particle counting

Accurately count high-spatial-density labeled molecular targets

3D PSF localization
Accurate photon counting
Drift correction
Cluster analysis

Deep Focus deconvolution

Enhance resolution & contrast of extended-depth images

Enhance resolution
Increase contrast
Reconstruct 3D volume from efficient axial sampling
Use GPU for accelerated performance

Data visualization and export available for all modules

3DTRAX.AI™

Upgrade to 3DTRAX.AI to use our top-performing Al models

3DTRAX.AI offers across-the-board improvements in:

PSF localization speed
PSF localization yield
PSF localization accuracy
Low-signal PSF localization
High-spatial-density PSF localization