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Double Helix Optics would like to thank Stephen Upton for his contributions to, and co-authoring of, this post. Figure 1. Reconstruction of bacteria imaged with PALM methodology with DHO’s SPINDLE module and DH2 Phase Mask. Image data is analyzed, processed, and rendered using DHO software, 3DTRAX®. This post highlights the work of researchers from the […]
Prof. Dan Schwartz from Colorado University Boulder presents his work using 3D DH-PSF tracking to study the dynamic behavior of 10,000’s of molecules in confined environments, such as at interfaces and within porous materials. This work has lead to validating long-time theories on the behavior of molecules at solid-liquid interfaces and advanced the understanding of […]
Professor Yoav Shechtman from Technion Israel Institute of Technology describes how deep learning, in combination with PSF engineering, enables unprecedented capabilities in super-resolution microscopy. Additionally, Professor Shechtman presents his recent work on combining PSF engineering with imaging flow cytometry.
Principal Investigator and Research Director, Dr. Valentina Emiliani of Institut of Vision presents work using holography with engineered phase masks for deep study of the brain and its circuitry.
"TILT3D enables low-background, 3D super-localization of single molecules on the surface of a cell as well as 3D super-resolution imaging inside of the cell. The TILT3D setup enabled the researchers to image and reconstruct structures throughout whole mammalian cells including mitochondria within the cell as well as the full nuclear lamina."