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Exploring The Unknown

Scientists are advancing their research with Double Helix Light Engineering.
Read their findings

Revealing Nanoscale Morphology of the Primary Cilium Using Super-Resolution Fluorescence Microscopy

Joshua Yoon, Colin J. Comerci, Lucien E. Weiss, Ljiljana Milenkovic, Tim Stearns, and W. E. Moerner, Biophys. J. 116, 319-329 (2019) (DOI: 10.1016/j.bpj.2018.11.3136, published online 7 December 2018).

Maximizing the field of view and accuracy in 3D Single Molecule Localization Microscopy

Rehman, S. A., Carr, A. R., Lenz, M. O., Lee, S. F., & O’Holleran, K.
Optics Express, 26(4), 4631. (2018).

3D single-molecule super-resolution microscopy with a tilted light sheet.

Gustavsson, A.-K., Petrov, P. N., Lee, M. Y., Shechtman, Y., & Moerner, W. E.
Communications 2018 9:1, 9(1), 123. (2018).

Three-Dimensional Tracking of Interfacial Hopping Diffusion.

Wang, D., Wu, H., & Schwartz, D. K.
Physical Review Letters, 119(26), 268001. (2017)

Real-time adaptive drift correction for super-resolution localization microscopy.

Grover, G., Mohrman, W. & Piestun, R.
Opt. Express 23, 23887–98 (2015).

Exploring bacterial cell biology with single-molecule tracking and super-resolution imaging.

Gahlmann, A. & Moerner, W. E
Nat. Rev. Microbiol. 12, 9–22 (2014).

Three-dimensional super-resolution and localization of dense clusters of single molecules.

Barsic, A., Grover, G. & Piestun, R.
Sci. Rep. 4, 5388 (2014).

The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging.

Backlund, M. P., Lew, M. D., Backer, A. S., Sahl, S. J. & Moerner, W. E.
Chemphyschem 15, 587–99 (2014).

Extending single-molecule microscopy using optical Fourier processing.

Backer, A. S. & Moerner, W. E.
J. Phys. Chem. B 118, 8313–29 (2014).

Small-molecule labeling of live cell surfaces for three-dimensional super-resolution microscopy.

Lee, M. K., Rai, P., Williams, J., Twieg, R. J. & Moerner, W. E.
J. Am. Chem. Soc. 136, 14003–6 (2014).

Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope.

Backlund, M. P., Joyner, R., Weis, K. & Moerner, W. E.
Mol. Biol. Cell 25, 3619–29 (2014).

Depth estimation and image recovery using broadband, incoherent illumination with engineered point spread functions [invited]

Jerod L. Ptacin, Andreas Gahlmann, Grant R. Bowman, Adam M. Perez, Alexander R. S. von Diezmann, Michael R. Eckart, W. E. Moerner, and Lucy Shapiro
Proc. Natl. Acad. Sci. U. S. A. 111, E2046–55 (2014).

Super-resolution fluorescence imaging with single molecules.

Sahl, S. J. & Moerner, W. E.
Curr. Opin. Struct. Biol. 23, 778–87 (2013).

The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision.

Lee, H.-L. D., Sahl, S. J., Lew, M. D. & Moerner, W. E.
Appl. Phys. Lett. 100, 153701–1537013 (2012).

Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE).

Grover, G., DeLuca, K., Quirin, S., DeLuca, J. & Piestun, R.
Opt. Express 20, 26681–95 (2012).

Limits of 3D dipole localization and orientation estimation for single-molecule imaging: towards Green’s tensor engineering.

Lee, H.-L. D., Sahl, S. J., Lew, M. D. & Moerner, W. E.
Appl. Phys. Lett. 100, 153701–1537013 (2012).

Simultaneous, accurate measurement of the 3D position and orientation of single molecules

Backlund, M. P. et al.
Proc. Natl. Acad. Sci. U. S. A. 109, 19087–92 (2012).

Three-dimensional parallel particle manipulation and tracking by integrating holographic optical tweezers and engineered point spread functions

Conkey, D. B., Trivedi, R. P., Pavani, S. R. P., Smalyukh, I. I. & Piestun, R
Opt. Express 19, 3835–42 (2011).

Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging.

Grover, G., Quirin, S., Fiedler, C. & Piestun, R.
Biomed. Opt. Express 2, 3010–20 (2011).

Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function.

Thompson, M. A., Casolari, J. M., Badieirostami, M., Brown, P. O. & Moerner, W. E.
Proc. Natl. Acad. Sci. U. S. A. 108, E1102–10 (2011)

Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane.

Badieirostami, M., Lew, M. D., Thompson, M. A. & Moerner, W. E.
Appl. Phys. Lett. 97, 161103 (2010).

Performance limits on three-dimensional particle localization in photon-limited microscopy.

Grover, G., Pavani, S. R. P. & Piestun, R.
Opt. Lett. 35, 3306–8 (2010).

In vivo Three-Dimensional Superresolution Fluorescence Tracking using a Double-Helix Point Spread Function.

Lew, M. D., Thompson, M. A., Badieirostami, M. & Moerner, W. E.
Proc. SPIE–the Int. Soc. Opt. Eng. 7571, 75710Z (2010).

Three-dimensional localization with nanometer accuracy using a detector-limited double-helix point spread function system.

Pavani, S. R. P., Greengard, A. & Piestun, R.
Appl. Phys. Lett. 95, 021103 (2009).

High-efficiency rotating point spread functions.

Pavani, S. R. P. & Piestun, R.
Opt. Express 16, 3484 (2008).

Depth from diffracted rotation.

Greengard, A., Schechner, Y. Y. & Piestun, R.
Opt. Lett. 31, 181 (2006).

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