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Exploring The Unknown

Scientists are advancing their research with Double Helix Light Engineering.
Read their findings

Publications

Electrostatic Barriers to Nanoparticle Accessibility of a Porous Matrix

Electrostatic Barriers to Nanoparticle Accessibility of a Porous MatrixHaichao Wu, Raphael Sarfati, Dapeng Wang, and Daniel K Schwartz Journal of the American Chemical Society Just Accepted Manuscript DOI: 10.1021/jacs.9b12096

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  • Deformation of Microgels at Solid–Liquid Interfaces Visualized in Three-Dimension

    L. H. Alvarez, S. Eisold, R. A. Gumerov, M. Strauch, A. A. Rudov, P. Lenssen, D. Merhof, I. I. Potemkin, U. Simon, and D. Wöll
    Nano Letters 2019 19 (12), 8862-8867DOI: 10.1021/acs.nanolett.9b03688

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  • Three-dimensional virtual refocusing of fluorescence microscopy images using deep learning

    Wu, Y., Rivenson, Y., Wang, H. et al. Three-dimensional virtual refocusing of fluorescence microscopy images using deep learning. Nat Methods (2019) doi:10.1038/s41592-019-0622-5

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  • Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules

    Rocha, J. M., Gahlmann, A. Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules. J. Vis. Exp. (151), e59387, doi:10.3791/59387 (2019).

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  • HER2 Cancer Protrusion Growth Signaling Regulated by Unhindered, Localized Filopodial Dynamics

    W. Lam, et al.; bioRxiv 654988; doi: https://doi.org/10.1101/654988

    HER2 Cancer Protrusion Growth Signaling Regulated by Unhindered, Localized Filopodial Dynamics

    W. Lam, et al.; bioRxiv 654988; doi: https://doi.org/10.1101/654988

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  • Metasurface integrated with double-helix point spread function and metalens for three-dimensional imaging

    Jin, C., Zhang, J. & Guo, C. (2019). Metasurface integrated with double-helix point spread function and metalens for three-dimensional imaging. Nanophotonics, 8(3), pp. 451-458. (2019) doi:10.1515/nanoph-2018-0216

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  • Diffusive Escape of a Nanoparticle from a Porous Cavity

    Dapeng Wang, Haichao Wu, Lijun Liu, Jizhong Chen, and Daniel K. Schwartz
    Phys. Rev. Lett. 123, 118002 (2019)

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  • Two-photon PSF-engineered image scanning microscopy

    Omer Tzang, Dan Feldkhun, Anurag Agrawal, Alexander Jesacher, and Rafael Piestun, “Two-photon PSF-engineered image scanning microscopy,” Opt. Lett. 44, 895-898 (2019)

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  • Quantitative Super-Resolution Microscopy of the Mammalian Glycocalyx

    L. Möckl, K. Pedram, A. R. Roy, V. Krishnan, A.-K. Gustavsson, O. Dorigo, C. R. Bertozzi, and W. Moerner, “Quantitative Super-Resolution Microscopy of the Mammalian Glycocalyx,” Developmental Cell, May 2019.

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  • Rapid 3D image scanning microscopy with multi-spot excitation and double-helix point spread function detection

    S. Li, J. Wu, H. Li, D. Lin, B. Yu, and J. Qu, “Rapid 3D image scanning microscopy with multi-spot excitation and double-helix point spread function detection,” Optics Express, vol. 26, no. 18, p. 23585, 2018.

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  • Deconvolution approach for 3D scanning microscopy with helical phase engineering

    Roider, Clemens; Heintzmann, Rainer; Piestun, Rafael; and Jesacher, Alexander, “Deconvolution approach for 3D scanning microscopy with helical phase engineering.” (2016). Electrical, Computer & Energy Engineering Faculty Contributions. 7.

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  • Chemistry of Photosensitive Fluorophores for Single-Molecule Localization Microscopy

    F. M. Jradi and L. D. Lavis, “Chemistry of Photosensitive Fluorophores for Single-Molecule Localization Microscopy,” ACS Chemical Biology, 2019.

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  • Spatio-temporal correlation super-resolution optical fluctuation imaging

    Purohit, Ashvini; Vandenberg, W.; Dertinger, Thomas; Wöll, Dominik; Dedecker, Peter; Enderlein, Jörg Spatio-temporal correlation super-resolution optical fluctuation imaging epl, 125, 2, 20005 (2019)
    [DOI: 10.1209/0295-5075/125/20005]

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  • Revealing Nanoscale Morphology of the Primary Cilium Using Super-Resolution Fluorescence Microscopy

    Joshua Yoon, Colin J. Comerci, Lucien E. Weiss, Ljiljana Milenkovic, Tim Stearns, and W. E. Moerner, Biophys. J. 116, 319-329 (2019) (DOI: 10.1016/j.bpj.2018.11.3136, published online 7 December 2018).

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  • Maximizing the field of view and accuracy in 3D Single Molecule Localization Microscopy

    Rehman, S. A., Carr, A. R., Lenz, M. O., Lee, S. F., & O’Holleran, K.
    Optics Express, 26(4), 4631. (2018).

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  • 3D single-molecule super-resolution microscopy with a tilted light sheet

    Gustavsson, A.-K., Petrov, P. N., Lee, M. Y., Shechtman, Y., & Moerner, W. E. 
    Communications 2018 9:1, 9(1), 123. (2018).

    3D single-molecule super-resolution microscopy with a tilted light sheet

    Gustavsson, A.-K., Petrov, P. N., Lee, M. Y., Shechtman, Y., & Moerner, W. E. 
    Communications 2018 9:1, 9(1), 123. (2018).

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  • Three-Dimensional Tracking of Interfacial Hopping Diffusion

    Wang, D., Wu, H., & Schwartz, D. K. 
    Physical Review Letters, 119(26), 268001. (2017)

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  • Enhanced information content for three-dimensional localization and tracking using the double-helix point spread function with variable-angle illumination epifluorescence microscopy

    Wang et al.
    Applied Physics Letters 110, 211107 (2017)

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  • Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function

    Carr et al. 
    BioPhysical Journal, 112, 1444-1454 (2017)

    Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function

    Carr et al. 
    BioPhysical Journal, 112, 1444-1454 (2017)

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  • ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure

    Jain, S. et al. 
    Cell 164, 487–498 (2016).

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  • Real-time adaptive drift correction for super-resolution localization microscopy

    Grover, G., Mohrman, W. & Piestun, R.
    Opt. Express 23, 23887–98 (2015).

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  • Exploring bacterial cell biology with single-molecule tracking and super-resolution imaging

    Gahlmann, A. & Moerner, W. E
    Nat. Rev. Microbiol. 12, 9–22 (2014).

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  • Three-dimensional super-resolution and localization of dense clusters of single molecules

    Barsic, A., Grover, G. & Piestun, R.
    Sci. Rep. 4, 5388 (2014).

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  • The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging

    Backlund, M. P., Lew, M. D., Backer, A. S., Sahl, S. J. & Moerner, W. E. 
    Chemphyschem 15, 587–99 (2014).

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  • Extending single-molecule microscopy using optical Fourier processing

    Backer, A. S. & Moerner, W. E. 
    J. Phys. Chem. B 118, 8313–29 (2014).

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  • Small-molecule labeling of live cell surfaces for three-dimensional super-resolution microscopy

    Lee, M. K., Rai, P., Williams, J., Twieg, R. J. & Moerner, W. E. 
    J. Am. Chem. Soc. 136, 14003–6 (2014).

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  • Correlations of three-dimensional motion of chromosomal loci in yeast revealed by the double-helix point spread function microscope

    Backlund, M. P., Joyner, R., Weis, K. & Moerner, W. E.
    Mol. Biol. Cell 25, 3619–29 (2014).

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  • Depth estimation and image recovery using broadband, incoherent illumination with engineered point spread functions [invited]

    Jerod L. Ptacin, Andreas Gahlmann, Grant R. Bowman, Adam M. Perez, Alexander R. S. von Diezmann, Michael R. Eckart, W. E. Moerner, and Lucy Shapiro 
    Proc. Natl. Acad. Sci. U. S. A. 111, E2046–55 (2014).

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  • The double-helix point spread function enables precise and accurate measurement of 3D single-molecule localization and orientation

    Backlund, M. P. et al. 
    SPIE–the Int. Soc. Opt. Eng. 8590, 85900 (2013).

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  • Super-resolution fluorescence imaging with single molecules

    Sahl, S. J. & Moerner, W. E. 
    Curr. Opin. Struct. Biol. 23, 778–87 (2013).

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  • Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions

    Gahlmann, A. et al.
    Nano Lett. 13, 987–93 (2013).

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  • Depth estimation and image recovery using broadband, incoherent illumination with engineered point spread functions [Invited]

    Quirin, S. & Piestun, R.
    Appl. Opt. 52, A367–76 (2013).

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  • Characterization of a three-dimensional double-helix point-spread function for fluorescence microscopy in the presence of spherical aberration

    Ghosh, S. & Preza, C.
    J. Biomed. Opt. 18, 036010 (2013).

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  • The double-helix microscope super-resolves extended biological structures by localizing single blinking molecules in three dimensions with nanoscale precision

    Lee, H.-L. D., Sahl, S. J., Lew, M. D. & Moerner, W. E.
    Appl. Phys. Lett. 100, 153701–1537013 (2012).

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  • Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE)

    Grover, G., DeLuca, K., Quirin, S., DeLuca, J. & Piestun, R.
    Opt. Express 20, 26681–95 (2012).

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  • Limits of 3D dipole localization and orientation estimation for single-molecule imaging: towards Green’s tensor engineering

    Lee, H.-L. D., Sahl, S. J., Lew, M. D. & Moerner, W. E.
    Appl. Phys. Lett. 100, 153701–1537013 (2012).

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  • Simultaneous, accurate measurement of the 3D position and orientation of single molecules

    Backlund, M. P. et al.
    Proc. Natl. Acad. Sci. U. S. A. 109, 19087–92 (2012).

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  • Three-dimensional parallel particle manipulation and tracking by integrating holographic optical tweezers and engineered point spread functions

    Conkey, D. B., Trivedi, R. P., Pavani, S. R. P., Smalyukh, I. I. & Piestun, R
    Opt. Express 19, 3835–42 (2011).

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  • Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging

    Grover, G., Quirin, S., Fiedler, C. & Piestun, R.
    Biomed. Opt. Express 2, 3010–20 (2011).

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  • Three-dimensional tracking of single mRNA particles in Saccharomyces cerevisiae using a double-helix point spread function

    Thompson, M. A., Casolari, J. M., Badieirostami, M., Brown, P. O. & Moerner, W. E.
    Proc. Natl. Acad. Sci. U. S. A. 108, E1102–10 (2011)

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  • Localizing and tracking single nanoscale emitters in three dimensions with high spatiotemporal resolution using a double-helix point spread function

    Thompson, M. A., Lew, M. D., Badieirostami, M. & Moerner, W. E. 
    Nano Lett. 10, 211–8 (2010).

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  • Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane

    Badieirostami, M., Lew, M. D., Thompson, M. A. & Moerner, W. E.
    Appl. Phys. Lett. 97, 161103 (2010).

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  • Performance limits on three-dimensional particle localization in photon-limited microscopy

    Grover, G., Pavani, S. R. P. & Piestun, R.
    Opt. Lett. 35, 3306–8 (2010).

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  • In vivo Three-Dimensional Superresolution Fluorescence Tracking using a Double-Helix Point Spread Function

    Lew, M. D., Thompson, M. A., Badieirostami, M. & Moerner, W. E.
    Proc. SPIE–the Int. Soc. Opt. Eng. 7571, 75710Z (2010).

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  • Three-dimensional localization with nanometer accuracy using a detector-limited double-helix point spread function system

    Pavani, S. R. P., Greengard, A. & Piestun, R.
    Appl. Phys. Lett. 95, 021103 (2009).

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  • Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function

    Pavani, S. R. P. et al.
    Proc. Natl. Acad. Sci. U. S. A. 106, 2995–9 (2009).

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  • Three dimensional tracking of fluorescent microparticles using a photon-limited double-helix response system

    Pavani, S. R. P. & Piestun, R.
    Opt. Express 16, 22048 (2008).

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  • High-efficiency rotating point spread functions

    Pavani, S. R. P. & Piestun, R.
    Opt. Express 16, 3484 (2008).

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  • Depth from diffracted rotation

    Greengard, A., Schechner, Y. Y. & Piestun, R.
    Opt. Lett. 31, 181 (2006).

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