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Carr et al. quantify the dynamics of T- cell receptors using extended depth 3D super-resolution imaging with Double Helix. This creative study combines the high-depth, high-precision Double Helix with multi-plane super-resolution imaging and single particle tracking to visualize the cell surface of entire live Eukaryotic cells.
Using the SPINDLE™, Wang et al showed that combining the Double Helix-PSF with variable-angle illumination epifluorescence microscopy (aka pseudo-TIRF), the signal to noise ratio, and hence, the localization precision of point emitters can be improved up to five-fold. This study will allow users of the SPINDLE™ to improve the accuracy of their image and track reconstructions with their current microscope set-up.
Jain and Wheeler et al. utilize the SPINDLE™ to visualize and characterize previously unseen stress granule cores. Stress granules are sub-cellular aggregates associated with ALS and Dementia. This ground-breaking study proposes a new model for the formation and dynamics of stress granules based on proteomic studies, in vitro isolation, and in vivo 3D Double Helix super-resolution imaging.
Gahlmann et al. utilize Double Helix with super-resolution to reconstruct three dimensional, three color super-resolution images of live bacteria using fluorescent proteins and a lipophilic dye. This innovative study quantitatively describes the sub-cellular structure of Caulobacter crescentus bacteria.
Grover et al. describe the Double Helix SPINDLE method and utilize super-resolution microscopy to reconstruct sub-diffraction 3D images of microtubules. This influential paper utilizes the Double Helix point spread function for high precision 3D sub diffraction super-resolution imaging.