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Double Helix Optics Blog

Imaging Eukaryotic Cells with the Double Helix Point Spread Function

In this blog posting, we would like to highlight research from the Lee Lab[1] at University of Cambridge using the Double Helix point spread function in the study of T-cells for whole-cell visualization, reconstruction, and 3D Tracking. In the article, titled  “Three-Dimensional Super-Resolution in Eukaryotic Cells Using the Double-Helix Point Spread Function,” researchers from the […]


isualization of large-scale reorganization of the outer membrane of Jurkat T cells on activating coated surfaces by mesh fitting to 3D localization data
3DTRAX Adds Overlapping Emitter Detection to 3D Single Molecule Localization and Tracking

Double Helix Optics announces the addition of the Overlapping Emitter detection algorithm to its Image J/Fiji-based 3DTRAX™ software for 3D single molucule localization microscopy (SMLM).  This latest version employs new algorithms and allows for emitters to overlap in the field of view, capturing more information from images and expanding what experimenters can do with sample preparation. […]


Three-Dimensional Tracking of Interfacial Hopping Diffusion

The three-dimensional movement of molecules in liquids is well described by Brownian motion, however, the movement of molecules near interfaces is not as well understood, especially in the case of how molecules move across the surface of the cell. The Schwartz Lab at the University of Colorado, Boulder, using Double Helix’s SPINDLE® imaging system, was […]


Tilted Light Sheet with DHO PSF Engineering for 3D Whole Cell and Sub-Cellular Super-Resolution Imaging

TILT3D enables low-background, 3D super-localization of single molecules on the surface of a cell as well as 3D super-resolution imaging inside of the cell.


Tilted Light Sheet examples

Older Posts:

Imaging Eukaryotic Cells with the Double Helix Point Spread Function
3DTRAX Adds Overlapping Emitter Detection to 3D Single Molecule Localization and Tracking
Three-Dimensional Tracking of Interfacial Hopping Diffusion
Tilted Light Sheet with DHO PSF Engineering for 3D Whole Cell and Sub-Cellular Super-Resolution Imaging